Study on Using Additive Manufacturing Technology to Fabricate Photo-Cured Multiple Biomaterial Scaffold for Tissue Engineering

• Main Authors: Pei-Chieh Wu, 吳沛頡
• Other Authors: Yih-Lin Cheng
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/x2gufe

碩士 === 國立臺灣科技大學 === 機械工程系 === 106 === At present, tissue engineering still uses single-material scaffold for vitro cell culture, but lack of the nutrients, oxygen and protein in cell growth causes the micro-environment can’t simulate the human body. By the combination of different material properties, a scaffold can be prepared to match different cell types and provide the functional environment. Therefore, this study established a multiple-material DLP system, research the bonding effect of different materials, and study on multi-material scaffold for cell culture. The DLP process is applied to produce a multiple-material scaffold with high precision and install a tank-changing mechanism is more convenient to change the material in the printing process to avoid the vibration of the machine.PGSA60+PCL-DA and PEG-DA were selected to test the difference in properties between the materials. The gap of interface and defect were found during the printing process, resulting in stress concentration and reduced loading capacity. It has been found that the curing depth of the bonding surface PEG-DA to 140 μm can effectively increase the bonding ability and print a precised scaffold with different materials.Through the implantation of Hepatocyte and HUVECs into the scaffold, it was found that the cells maintained good biocompatibility during the culture. In addition, in order to increase the stability of vascular material PEG-DA immersion in water and the ability of molecular diffusion, PGSA has been added to formulate, and it is expected that the process and materials will be more widely application in in the tissue engineering.