Synthesis of porphyrin-conjugated confeito-like gold nanoparticles for photodynamic therapy

• Main Author: Do Thi Anh Thu
• Other Authors: Toyoko Imae
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/6q34jb

碩士 === 國立臺灣科技大學 === 應用科技研究所 === 106 === Gold nanoparticles (AuNPs) possess the characteristic of both inertness and biocompatibility which have a great medical application in the biosensor and drug delivery. When AuNPs with 10-100 nm in size are exposed to optical radiation, the electrons within the AuNPs resonate, causing them to absorb and scatter the light (called the surface plasmon resonance). In biomedicine, this unique optical property can be exploited for application such as photodynamic therapy. In this study, confeito-like gold nanoparticles were synthesized by green chemistry method. UV-vis absorption spectroscopy and transmission electron microscopy were used to characterization of confeito-like AuNPs. This study aims the synthesis of porphyrin-conjugated confeito-like AuNPs (Au@Cys@TCPP and Au@FeC@TCPP) for photodynamic therapy (PDT) application. They were prepared by covalent binding, with using L-cysteine and carbon dots-protected iron oxide (FeC or Fe3O¬4@C) as adapted linkages. For L-cysteine, the thiol group of L-cysteine was bound to confeito-like AuNPs and also formed amide bonding between porphyrin TCPP and L-cysteine to obtained Au@Cys@TCPP which was confirmed by FT-IR spectroscopy. On the other hand, co-precipitation procedure was the method to obtain magnetic Fe3O4 particles and the hydrothermal procedure was used for the synthesis of Fe3O4@C. Fe¬3O4@C has carboxylic and amine groups which can be formed the amide bonding between confeito-like AuNPs and porphyrin TCPP. Both of porphyrin-conjugated confeito-like AuNPs were characterized by using FT-IR spectroscopy, UV-vis spectroscopy and transmission electron microscopy. The effectiveness of the porphyrin-conjugated confeito-like gold nanoparticles was evaluated by the singlet oxygen generation. These results indicate a highly potential of not only porphyrin-conjugated confeito-like AuNPs but also confeito-like AuNPs for PDT.