| Summary: | 碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 106 === Aim : The aim of the study is to investigate the differentiation potentials of human apical papilla cells induced by TGF-β1 by analyzing the expressions of differentiation related markers, including osterix (osx), nestin, vimentin, connective tissue growth factor (CTGF), N-cadherin, cementum protein 1 (CEMP1), cementum attachment protein(CAP), and plasminogen activation inhibitor-1(PAI-1). The signaling pathway conveying the effects was also explored.
Materials and methods: Primary-cultured human apical papilla cells were treated with different concentrations (0, 0.5, 1, 5, 10, 25 ng/ml) of TGF-β1 for 24 hours. The expressions of osx, nestin, vimentin, CTGF, N-cadherin, CEMP1, CAP, and PAI-1 were evaluated through RT-PCR, western blot, and immunofluorescence. In addition, pretreatment of SB431542 (as a specific ALK5/Smad2 inhibitor) was used to investigate the Smad-dependent (canonical) pathway in TGF-β1-induced effects on human apical papilla cells.
Results: Human apical papilla cells exposed to TGF-β1 for 24 hours showed no obvious morphology change. The mRNA and/or protein expressions of osx, nestin, vimentin, CTGF, N-cadherin, CEMP1, CAP, and PAI-1 showed increased when adding TGF-β1. Besides, pretreatment of 1and 2.5 µM SB431542 reduced TGF-β1 stimulated-expressions of nestin, vimentin, CTGF, N-cadherin, and PAI-1. However, the expressions of osx, CEMP1, and CAP were not inhibited by adding inhibitor SB431542.
Conclusions : TGF-β1 may be capable to induce various differentiation potentials of human apical papilla cells such as osteogenesis, odontogenesis and cementogenesis, and to modulate the turnover of extracellular matrix. The expressions of nestin, vimentin, CTGF, N-cadherin, and PAI-1 induced by TGF-β1 were conveyed through ALK5/Smad2 signaling pathway. However, the expressions of Osx, CEMP1, and CAP induced by TGF-β1 were not. The information may benefit the development of new strategies for regenerative endodontics.
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