| Summary: | 碩士 === 國立臺灣大學 === 臨床動物醫學研究所 === 106 === The healing of bone tissuehas always been a popular researchtopic. Besides restorationof clinical functionand returning to a macroscopic normal anatomical structure, changes at the microscopic cellular and molecular level should also be investigated. Heparin sulfate proteoglycans(HSPGs) are found on cell surfaceand in extracellularmatrix, where they participatein many signaling pathways of different growth factors. It is postulated thatmodulating HSPGs may influencethe processof bone healing indirectly.Heparinase III derived from microorganismiscapable of cleavingthe heparansulfate (HS) chain on HSPGs. This study aims to evaluate the effectof heparinase III in bone healing in mouse model. Mice with a distal femur bone defect were used as the animal model. Mice were randomly assigned to either the control group receiving heparinase buffer or the experiment group receiving heparinase III in heparinase buffer. The mice were sacrificed at1, 4, 7, 14, 21 days post-injury, and micro-CT, histological exam, qPCR, and immunohistochemistrywere performedto evaluate bone healing. Results showedthat the main difference betweenthe two groups are acid phosphatase histochemistry results at 1 day post-injury and Cd68 qPCR results at 4 days post-injury, with heparinase III group showing significant higher osteoclastnumbers and distribution. These results indicate that heparinase III may not affectthe final outcomeof bone healing, but certainly play a role in the early stage of osteoclastrecruitmentand bone resorption.
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