Polymorphism of Interleukin-10 gene in Gestational Diabetes Mellitus

• Main Authors: Chien-Wen Yang, 楊茜雯
• Other Authors: Shin-Yu Lin
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/y4n3fy

碩士 === 國立臺灣大學 === 分子醫學研究所 === 106 === Gestational diabetes mellitus (GDM) is the most common metabolic disease in pregnant women. Interleukin-10 (IL-10) is an important anti-inflammatory cytokines for pregnancy maintenance and development. Previous studies indicated that three IL-10 promoter region single nucleotide polymorphisms (SNPs) at position -592 C>A (rs1800872), -819 C>T (rs1800871), and -1082 A>G (rs1800896) play an important role in IL-10 secretion capacity, but it’s genetic polymorphisms in GDM are unknown. In addition to the three common SNPs -592, -819, and -1082, we also explore a rare to be reported position 3388 A>C (rs3021094). We examined whether these SNPs variant are associated with IL-10 production and GDM susceptibility. Our case-control study included 100 healthy controls and 42 women with GDM grouped according to 75g oral glucose tolerance tests (OGTT). The polymorphisms of genotype and allele frequencies were analyzed by Chi-square test or Fisher’s exact test. Odds ratio (OR) and 95% confidence interval (CI) were used to explain relative risk of GDM. Maternal plasma IL-10 levels were measured by ELISA and associated with the polymorphisms of genotype were analyzed by non-parametric Kruskal-Wallis H test or Mann Whitney U test. In the dominant model (AA+CA versus CC) of IL-10 3388 A>C, AA+CA genotype and A allele frequencies were significantly higher in cases compared with controls (P < 0.05), suggesting significant association with risk of GDM (OR = 2.88, 95% CI [1.03-8.07]; OR = 1.85, 95%CI [1.10-3.12]). No significant difference of -592 C>A, -819 C>T, and -1082 A>G were observed between cases and controls (P > 0.05). CC genotype of -592 C>A, CC genotype of -819 C>T, and AA genotype of 3388 A>C were all significantly higher IL-10 plasma level production compared with other genotypes (P < 0.05), suggesting significant association with IL-10 high-level production. No significant difference of -1082 A>G were observed (P > 0.05). In conclusion, SNP at position IL-10 3388 A>C was significantly associated with the risk of GDM and IL-10 plasma level high production. IL-10 3388 A>C has potential for use as a predictive maker for GDM.