| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 106 === Combination therapy has become a new trend in oncology since its potential to improve treatment response, minimize development of resistance or adverse events. Herein, we report a nanoparticle encapsulate two anti-cancer drugs to achieve synergic therapeutic efficiency of photodynamic therapy and chemotherapy through two-photon laser. Due to its high bio-compatibility and excellent bio-degeadability, mPEG-PCL was utilized as drug delivery vehicle material. Doxorubicin (DOX) and sunitinib (sutent) were both encapsulated in empty mPEG-PCL micelles as sutent/DOX NPs and achieve Enhanced Permeability and Retention (EPR) effect to accumulate in tumor. As to the results, the sutent/DOX NPs were with an average diameter around 110nm and the encapsulated efficiency were more than 60%. Morphology was further confirmed by TEM image. In addition, sutent/DOX NPs showed a great ability to generate ROS after exposed to a specific region of laser. However, obstacles for the region of laser to activate sutent is with low permeability and not ease to achieve the tumor. Two-photon laser was utilized in the experiment according to its high permeability compare to the normal 430nm laser. MCF-7 cells and MCF-7/ADR cells were utilized in the in vitro experiments. Experiments about DOX IC50 was measured to ensure the drug resistance of MCF-7/ADR cells. Regarding to the cell viability test, sutent/DOX NPs triggerd by laser were quite efficient to kill the MCF-7/ADR cells. As a result, the co-loaded nanoparticles would simultaneously achieve PDT treatment and chemotherapy effect to kill drug-resistant cancer cells.
|
|---|
