Early diagnosis of acute mesenteric ischemia based on multi-photon fluorescence microscopy

• Main Authors: Po-Lun Yang, 楊博綸
• Other Authors: Tzu-Ming Liu
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/8d9etz

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 106 === We have observed the yellow fluorescence of mesenteric ischemia, and ischemia-reperfusion rats with multi-photon fluorescence lifetime and intensity, which based on a femtosecond Ti:sapphire laser. With lifetime trace, single photon, two-photon fluorescence spectrum, and two-photon fluorescence excitation spectrum, we found out that intensity of yellow fluorescence increases during ischemia state, and recovered gradually after reperfusion. The characteristic change of the autofluorescence intensity ratio in two-photon excitation spectrum, which observed only in the ischemia state instead of normal state or reperfusion state. We compared fluorescence decay traces of preishemia, ischemia state, and ischemia-reperfusion state, the results showed these traces are almost overlapping, which indicated that there is no any new fluorescent substance producing during the whole process. From above results, the elevating intensity of ischemia state is due to the concentration of original fluorescent substances increase. We tried to identify that fluorescent substance by High-performance Liquid Chromatography-Mass Spectrophotometer (HPLC-MS), and the preliminary result is riboflavin. With our study result, we believe two-photon fluorescence excitation spectrum, a non-invasive way, is potential to serve as a tool for early diagnosis of acute mesenteric ischemia.