Root Transcriptome Analysis of Transgenic Rice Overexpressing OsbHLH068 Transcription Factor

• Main Authors: Tsung-Yang Chen, 陳宗暘
• Other Authors: 張孟基
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/79f55p

碩士 === 國立臺灣大學 === 農藝學研究所 === 106 === Transcription factors regulate plant growth, development and response to various stresses. The basic/helix-loop-helix (bHLH) transcription factor belongs to a large rice transcription factor gene family. However, The functions of OsbHLHs gene involved in rice growth and development are not well studied. From public and laboratory microarray data, we picked up OsbHLH068 which is induced by salt for further study. Functional analysis of OsbHLH068 gene showed that heterologous over-expression OsbHLH068 in Arabidopsis thaliana, results in enhanced root elongation and delayed flowering time. Similar results were also obtained in overexpressed OsbHLH068 transgenic rice which displayed longer root phenotype. However, the regulation of OsbHLH068 in root elongation is not clear. To find out the possible molecular mechanism, we used RNA-sequencing technology and conducted transcriptome analysis of over-expressed transgenic rice root. The results showed that there are 467 differentially expressed genes (DEGs), including 212 up-regulated genes and 255 down-regulated genes. With assistance of Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genome (KEGG), these genes can be categorized into various groups that mainly involved in stress stimulation reaction, lipid transport, and hormone signal transduction. From the results, root growth affected by OsbHLH068 may related to the regulation of Auxin, Cytokinin and Jasmonate signal transduction. Interestingly, RNA-Seq data and qPCR demonstrated that OsPME1 and OsEXPA7 genes were up-regulated in over-expressed OsbHLH068 rice. Taken together, this study showed that OsbHLH068 may not only involved in salinity stress response but also participate in regulating rice root growth.