| Summary: | 博士 === 國立臺灣大學 === 化學研究所 === 106 === We developed new design intracellular pH (pHi) sensing probes by using available nanomaterials, quantum dots and Mesoporous Silica Nanoparticles. First, we synthesized single Mn2+doped quantum dots (DPA-MnQdots) which exhibit 2 emissions and show reliable and general features of common ratiometric sensing based on 2 fluorophores. The changing from “two” to “one” will simplify ratiometric sensing design and promote the corresponding applications like imaging. The biocompatible DPA-MnQdots were applied for long-term monitoring of local microenvironments pH values during endocytosis in HeLa cells. Second, we synthesis dye-loaded fluorescent mesoporous silica nanoparticles decorated lysosomal sorting peptide (YXXɸ), called peptide(YXXɸ)-FORMSN, potentially act as drug carrier to treat Lysosomal Storage Diseases as well as pHi sensing probe. This approach allowed long-term reliable ratiometric pH measurements with precision better than 0.3 pH units and broaden the window of pH value detection. Owing to its excellent brightness and stability, the FORMSN could meet the criteria to serve as a single particle tracking (SPT) probe. In addition, super-resolution 3D SPT with a simple marker-assisted drift correction, was also developed to visualize the motion and record the local pH value of individual peptide-FORMSN during its journey with high-precision. The overall data from fluctuation of local pH value surrounding of peptide-FORMSNs, real-time trajectory, and real-time speed variation represent the dynamic interactions of peptide-FORMSNs with their cellular hosts during their entire journey. As result, we were able to construct the whole pathway, traditional and alternative route, of peptide -FORMSNs for targeting lysosome.
|
|---|
