The effects of nano-silver on the movement patterns of planarians

• Main Authors: Shun-Lung Chang, 張順龍
• Other Authors: Wei-Chen Liao
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/g2xftq

碩士 === 國立臺東大學 === 應用科學系 === 105 === The purpose of this study is to examine the toxicity of silver nanoparticles (AgNPs) in the regenerating planarians. After the AgNPs treatments, the planarian locomotion data were collected and analyzed. A standardized circular pathways platform was developed to quantify and profiling the locomotion patterns of planarians. The standardized circular pathways platform was designed to force planarian moving forward, however, experiments showed that the platform still allow planarian moving clockwise or counterclockwise. We defined the “looking around” behavior as explore time (ET). The explore time was increased both the AgNPs treatments groups and planarian cutting group. The performance of planarians in circular pathways shown that the moving direction of the control group was counterclockwise , however, the planarian cutting group changed the direction clockwise. After the AgNPs treatments, the planarian moving direction was restored to counterclockwise-oriented mode . We developed a novel approach for the locomotion testing of planarian using circular orbit in a limited space. The method, we named it “Planarian Circular Orbit LocoMotion Velocity (pCOLMV)”, can be quite useful in biomedical research, especially in the drug development for regeneration medicine.