Using photosynthetic ferredoxin inducible Bacillus spp. to increase the resistance to high temperature, UV and Ralstonia solanacearum in Capsicum annuum Linn. and Lycopersicon esculentum Mill.

• Main Authors: Chih Hsuan Huang, 黃志暄
• Other Authors: Hsiang En Huang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/4gxz3k

碩士 === 國立臺東大學 === 生命科學系碩士班 === 105 === Plant grown in the natural environment often suffer many stresses and by changing the expression of the plant photosynthetic type ferredoxin (PT-Fd) increasing plant resistance to stresses could effectively improve the yield and quality of plants. A previous study revealed that overexpression of PT-Fd by the transgenic technology can increase plant resistance to abiotic and biotic stresses. However, due to the unknow safty concern, the transgenic plant are generally not accepted. In order to reduce the controversies using a transgenic plants, Bacillus spp, which has been widely used as a biological control agent, wese screened in this study. The results showed that three rhizobacterium can induce PT-Fd expression in pepper (Capsicum annuum Linn.) and tomato (Lycopersicon esculentum Mill.), namely as HS1, HS2 and HS3. Sequences of the 16S rDNA revealed that HS1, HS2 and HS3 are B. thuringiensis, B. subtilis or B. cereus as well as B. amyloliquefaciens, respectively. Only HS2 has the ability to increase the leaf size, plant height and root development of pepper. H2O2 and MDA were accumulated after HS1 and HS2 treated, but POD was not. We also found that PR1 gene in pepper was strongly induced after 30-day-old plants were treated with 107 CFU/g of HS1, HS2 and HS3. Higher resistance against R. solanacearum Rd4 and expressing of PR1 gene could be observed in the HS1, HS2 and HS3 pre-treated pepper plants. Furthermore, the application of HS1 and HS2 in soil would increase pepper tolerance to high temperature and UV-C (19W). The results indicated that application of rhizobacteria can achieve the same goal as use of transgenic plants to overexpress PT-Fd. In the future, such strategy can be applied to the situation where regulation of genes in basic metabolic pathwat such as PT-Fd can be used to develop molecular markers for screening soil microorganisms with the ability to protect plants.