Curcumin Loaded Mesoporous Silica Nanoparticles with dual-imaging and temperature control inhibits the infection of Zika virus

• Main Authors: Lo, Tzu-Hsuan, 羅子軒
• Other Authors: Lin, Hsiu-Mei
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/uahyb2

碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 106 === Zika virus is a virus which spread by mosquitoes or vertical transmission. If the woman who is pregnant and infected by Zika, the newborn baby will have microcephaly. Currently there are no specific drugs or vaccine, we can only use supportive therapy to treat the infection of Zika. Curcumin is a common polyphenol compound used in cooking and has been found to have anti-inflammatory, anti-cancer, anti-oxidant and anti-viral activity effects. However, curcumin is hardly soluble in water and has poor stability, poor biocompatibility, poor drug metabolism and other shortcomings. These reason caused curcumin is not easy to use for medical treatment. Mesoporous silica nanoparticles (MSN) have good biocompatibility. It can be modified molecules on surface and loaded drugs into its pores. This experiment add the metal in the preparation of MSN to make it have dual-imaging function and modifies the molecules with lower critical solution temperature on the surface, because Zika infection will lead to fever. Through the above steps, the loaded curcumin can be controlled release by temperature. These will increase curcumin’s biocompatibility, stability and bioavailability, etc., to treat the infection of Zika virus. The experiment will be divided into two parts. In the first part, mesoporous silica nanoparticles with dual-imaging function and temperature-controlled release and loaded curcumin (MSN-EuGd-PEGMA@Cur) were synthesized. First, we add metal to doped silica nanoparticles. After the assembly is formed, mesoporous silica nanoparticles with dual-imaging function (MSN-EuGd) can be obtained by calcining to removing the template. The synthesized MSN-EuGd will be modified with PEOMA on surface and loaded with curcumin. The second part is comparing curcumin and MSN-EuGd-PEGMA@Cur for the ability of anti-virus and its dual-imaging function and temperature control function.