| Summary: | 碩士 === 國立臺灣海洋大學 === 機械與機電工程學系 === 106 === Colorectal cancer is the collective name for colorectal cancer. It ranks among the top three causes of cancer death in China. It is mainly due to the lack of regular exercise in the busy life of modern people, and it is loved to eat high-fat, high-cholesterol or low-fiber foods, and its incidence has increased year by year. At present, the main methods for treating cancer are surgical resection, radiation or chemical treatment. Although the first two methods can kill cancer cells, they also have an effect on normal cells, which may easily cause side effects. Therefore, this study hopes to provide a system with good stability and rapid screening of chemotherapy drugs, which can record the growth history and inhibition process of colon cancer cells, and use the results as a basis for drug screening, and then develop more suitable chemotherapy for human body. drug.
This study integrates microelectromechanical process technology and PDMS microchannel packaging technology, and was commissioned by Taiwan Semiconductor Manufacturing Co., Ltd. to produce piezoresistive miniature cantilever beam wafers and successfully integrated into the drug fast screening system. The system is 31.5cm long, 20cm wide and 26cm high. It integrates constant temperature, electromechanical control, automatic cleaning and UV lamp sterilization. It has the characteristics of light weight, small size and convenient carrying. In this system, only 0.6 mL of test samples can be placed into the system. Users can control the operation of the system by the human interface of the system, which can greatly reduce the human error. The LCD screen on this interface can be used. Provide user temperature control and process status information, to facilitate users to understand the current situation and to give appropriate treatment, and finally record the resistance change of the piezoresistive cantilever beam with a digital meter to analyze the association between drugs and cancer cells.
It was confirmed by heating experiments that the design of the thermostatic mechanism of this study allowed the system to reach a preset temperature after about 30 minutes of opening and to maintain stability for a long time. And through related experiments such as E. coli, it is confirmed that the UV lamp can be turned on for more than 20 minutes before using the system, so that the sterilization effect can be achieved inside the system, so that CT26.WT cancer cells can survive in the microcavity for a long time, avoiding being subjected to external The pollution affects the accuracy of the experiment. In addition, when the 5-FU chemotherapeutic drug is added, the recorded resistance change graph shows a two-stage change. The first phase change is the unstable state of the fluid generated when the drug is injected. After verification, it is known that after 40 minutes, Stable, the second phase of change is the effect of drugs on the inhibition or poisoning of CT26.WT cancer cells. The overall measurement time includes cell attachment and drug screening for about 6-7 hours, and the numerical analysis can be completed in one day. Understand whether the drug has an inhibitory or killing effect on cancer cells.
The chemotherapeutic drug fast screening system has the advantages of rapid analysis, instant detection, and lightweight portable, which can greatly reduce the time from the research and development to the clinical stage of chemotherapy drugs, and is a great contribution to the social and national medical technology.
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