Population structure of mangrove red snapper Lutjanus argentimaculatus revealed by growth parameter and microsatellite DNA around Taiwan

• Main Authors: Jiang, Wan Jhen, 蔣宛臻
• Other Authors: Shen, Kang-Ning
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/znqa7w

碩士 === 國立臺灣海洋大學 === 環境生物與漁業科學學系 === 106 === The mangrove red snapper (Lutjanus argentimaculatus) is widely distributed in tropical and sub-tropical Indo-West Pacific. It is an econonmic and valuable species in Taiwan. Taiwan is located in subtropical regions and surrounded by the ocean, the fisheries resource is highly diversed. In recent years, the industry developed and development of fishery technology casued fishery resource decreased. Releasing hatchery fish fry is one of the most effective methods for sea ranching. However, the studies of wild population structure need long-term evaluation. In this study, growth parameter and genetical analytical methods were applied to analyze mangrove red snapper (Lutjanus argentimaculatus) population structure around Taiwan. A total of 405 specimens were collected from northeast, northern, central, southern Taiwan and Penghu between March 2015 and July 2016. Total length of each individuals were measured, and biological tissue and sagittal otolith were collected. Age of 278 fish was read from otolith sections. Otolith opaque zones appeared on the edge from April to July, and the annulus forms one ring per year. The results showed that the age structure of the 2 - year - old samples were the most abundant, accounting for 39%, of which 1 - 7 years old accounted for nearly 95%. The results showed a significant difference between the northeastern and southern regions (F1,188= 6.503, p < 0.05), and in the Phi primer test calculation, the values is range from 0.0 to 0.4 in the five range. Based on the data collected from the samples collected in this study, there may be differences between individuals in the wild population of mangrove red snapper around Taiwan, but whether it has been diversed into populations need further investigate. We have also developed 16 microsatellite loci primers by using NGS for further population structure investigation. The genotyping results from 4 microsatellite loci showed that there was no significant difference among the wild populations, but only the central region (Yunlin) in 2016 (p < 0.001). However, aquaculture populations are significant different to wild populations (all p < 0.005). The results suggest that the otolith growth parameters and microsatellite genotypes are feasible in the analysis of the wild population structure of mangrove red snapper.