| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 106 === The simultaneous determination of 23 artificial dyes in dried fish, fish roe and dried shrimp by using high performance liquid chromatography coupled to diode array detector and quadrupole orbitrap mass spectrometry (HPLC-DAD-Q-Orbitrap) was developed in this study. The samples were homogenized and extracted by water, methanol and tetrahydrofuran. Transesterification with sodium methoxide to change triglycerides converted to fatty acid methyl esters. Organic phase was SPE by Silica gel SepPak plus silica column to extracted oil-soluble dyes. Water phase was SPE by Oasis PRiME hydrophilic-lipophilic-balanced column after adjusted pH value to 4 with 5 N hydrochloric acid. The extracts wrer chromatographed by Acquity UPLC BEH C18 column (2.1 mm × 100 mm,1.7 μm) with mobile phase solution of 40 mM ammonium acetate and 2.5% acetonitrile (pH 7.8). The detecting wavelengths of 427, 500, 550 and 620 nm were used and the retention time was 23 minutes for each sample. Ionizing collision energy were 30 and 60 eV. Detection mode was under full scan m/z 70 to 1050. All peaks of chromatography showed good resolution. The recovery rates of each artificial dye in dried fish product ranged from 99.6% to 52.5% with coefficient variation being less than 12.3%. The relationship of concentration of each analyte and responding peak was linear between the range of 0.005 - 1 mg/kg, and all coefficient values are greater than 0.995. It means the developed method are useful for determining 23 artificial dyes in dried fish, fish roe and dried shrimp products. Furthermore, 15 dried fish, 12 dried shrimps and 12 fish roe products were collected from Taipei, New Taipei, Keelung and Kaohsiung for artificial dye analysis. It was found that 3 dried fish products contained legal artificial dye. One fish roe sample contained legal artificial dye, 1 sample contained illegal artificial dye Azorubine and 2 samples contained illegal artificial dye Orange II. All dried shrimp contained more than 1 artificial dyes and 2 dried shrimp samples contained illegal artificial dye Orange II. Result indicated that developed method can apply to artificial dyes detected in dried fish, fish roe and dried shrimp sample.
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