Investigation of anti-hepatocellular carcinoma agent, Chinese herbal medicine M1

• Main Authors: Fu, Yi-Yan, 傅乙晏
• Other Authors: Lin, Jung-Yaw
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/5699n7

碩士 === 國立臺灣師範大學 === 生命科學系 === 106 === Hepatocellular Carcinoma (HCC) is one of higher malignant in cancers of human in the world. In addition, HCC mortality rate for 20 years is the leading cause of cancer death in Taiwan. The primary carcinogenic factors includes hepatitis virus infection and other are caused by alcohol abuse, and ingestion of carcinogen such as aflatoxin, etc. HCC has the characteristics of high level metastasis, angiogenesis, and recurrence after surgery. In this study, we focused on how to effectively prevent metastasis and angiogenesis of HCC. Recently, it becomes important to use the Chinese Herbal Medicine (CHM) as a therapeutic strategy for the treatment of diseases. Here, we found that among 10 kinds of CHMs, M1 could inhibit the cell migration at the dose less than half concentration of IC50 (the half maximal inhibitory of concentration). M1 significantly inhibited the expression of Receptor Tyrosine Kinase, Epidermal Growth Factor Receptor (EGFR), and downstream, Ras, ERK, and Snail. Furthermore, another downstream pathway protein, Protein Kinase B (AKT), mammalian target of rapamycin (mTOR), Hypoxia-inducible factor 1-alpha (HIF1-α), and Vascular Endothelial Growth Factor A (VEGFA) were also inhibited. And the Matrix Metalloproteinases (MMPs), including MMP-2, MMP-9, which play an important role in metastasis mechanism, were also inhibited by M1. Epithelial–mesenchymal transition (EMT) markers, such as E-cadherin and N-cadherin, were effective altered by M1 treatment. In the xenograft mice model, we observed the tumors growth sizes, weight, and protein expressions were effectively inhibited by M1. In conclusions, we demonstrate that M1 has an inhibitory effect to suppress angiogenesis and metastasis of HCC in vitro and in vivo.