| Summary: | 碩士 === 國立清華大學 === 生醫工程與環境科學系 === 106 === Environmental pollution caused by chromium (Cr) is closely related to the industrial activities, such as steel, leather, pigment and rubber industries. Typically, Cr exists either as cationic trivalent species (i.e., Cr(III)) or anionic hexavalent species (i.e., Cr(VI)). Cr(III) species are essential in protein and lipid metabolism. In contrast, Cr(VI) species are capable of inducing deoxyribonucleic acid (DNA) damage leading to potential cell transforming effects. Considering Cr(III) species are prone to form more toxic Cr(VI) species in the environment, the determination of Cr(VI) is critical for evaluating their impacts on the environment and human being.
Over past decades, the microfluidic-based technology has emerged as a popular methodology for analytical work due to their unique advantages such as low reagent/power consumption, portability for in-situ use, low fabrication cost, and versatile design. Among several microfluidic-based techniques available, centrifugal microdevices have attracted much attention in recent years. Compared to the usual methods for fluid manipulation in miniaturized systems, the sample/reagent solutions are driven by centrifugal force without the use of any other pumping equipment. Thus, more compact designs for analytical systems can be expected.
In this study, we developed a centrifugal device including a glucamine-packed solid phase extraction column and in-channel flow control valves. After coupling the developed centrifugal device to simple image analysis tools. Under the optimized condition, it merely needed 15 minutes to analyze six samples in a single run. The limit of detection of Cr(VI) were 1.69 mg L─1. Based on the analytical results, the proposed system was proven to be a promising platform for determination of Cr(VI).
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