The Synthesis and Structure Stability of Stapled Polyproline Peptides

• Main Authors: Tseng, Wen-Hsiu, 曾文秀
• Other Authors: Wang, Sheng-Kai
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/z59psm

碩士 === 國立清華大學 === 化學系所 === 106 === In recent years, many drugs and carriers based on peptides has been developed. However, drug design based on peptide has a critical disadvantage that they may be degraded by enzymes in organism. For this issue, peptide stapling is one practical solution to extend the lifespan of peptides. While polyproline peptides have unique structure for various applications, we “stapled” polyproline peptide systematically to investigate the effects on polyproline helix II (PP II) structure by change linker length and the locations of stapled residues. From circular dischroism (CD) analysis, we found that stapling at i, i+3 residues of polyproline peptides stabilizing PP II structure better than other locations at i, i+1 and i, i+2. Compared to normal polyproline, the PP II structure stapled polyproline is also affected by the staple length in n-propanol. In addition to CD spectrum, we use GROMACS software to simulate the molecular dynamics of stapled peptides in water.