Studies on the cell cycle regulation roles of GREM1 gene inBFTC905 cells

• Main Authors: Wei-Ru Huang, 黃瑋茹
• Other Authors: Yow-Ling Shiue
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/2b5fnf

碩士 === 國立中山大學 === 生物醫學研究所 === 106 === Urinary bladder urothelial carcinoma (UBUC) is the second largest cancer in urine system. It is hard to cure and highly recurrent so it costs lots of time and money to completely cure. Risk factor of UBUC includes occupational exposure and disease inducing. In recent years, human whole genome screening is universal. There is a discovery of relation between abnormal gene and bladder cancer due to the generality of data mining that GREM1 has abnormally high expression in cells of patients which suffer from UBUC. GREM1 is rarely studied in bladder cancer. In this study, we explored the relation between GREM1 and cell proliferation of UBUC. Overexpression of GREM1 in cells induced G0/G1 cell cycle arrest, increased CKIs protein expression of control cell cycle, and downregulated cell migration. In contrast, when downregulation of GREM1 in BFTC905 bladder cancer cells, it accelerated cell cycle S phase, cell proliferation, anchorage-independent cell growth, cell migration, invasion, and inhibited CKIs protein expression of control cell cycle cell. Therefore, our data indicated that GREM1 functions as a tumor suppressor in bladder cancer by inducing G0/G1 cycle arrest and downregulating cell growth.