| Summary: | 碩士 === 國立中山大學 === 生物醫學研究所 === 106 === Head and neck squamous cell carcinoma (HNSCC) is the sixth most common type of cancer worldwide,including the oral cavity, pharynx, and larynx ,with more than 600,000 new cases reported each year. The incidence of oral cancer ranked fifth, and the mortality rate ranked fourth of top 10 cancer in Taiwan. Accroding to previous study, the extract of the whole plant Thelypteris torresiana (Gaud.) has the select killing effect and induce autophagy on oral cancer cell in vitro.AN30 has been tested in many cancers except in oral cancer. In this study, we tested and summarized the effects concerning about ROS, apoptosis and autophagy of AN30 treatment on oral cancer cells. The results show levels of ROS and apoptosis are raised in dose dependent mode. After NAC treatment, the level of apoptosis is decreased in annexin V and pan-caspase flow cytometry assay. We also analyze gene expression of cancer cells under AN30 treatment with bioinformation and microarray data, and found FoxM1, TXNRD1 expressions are induced by ROS, DUSP1 expression decreases under autophagy, and MAP2K6, MYLK expression decreases under apoptosis. These results will provide bases for further application of AN30 on cancer therapy.
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