Synthesis of Lysozyme-Stabilized Gold Nanoclusters for Fluorescent and Naked-Eye Detection of Cyanide.

• Main Authors: Hung-Chi Chiu, 邱虹綺
• Other Authors: Wei-Lung Tseng
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/th744d

碩士 === 國立中山大學 === 化學系研究所 === 106 === This study developed a simple strategy to prepare lysozyme nanoparticle-encapsulated gold nanoclusters (LysNP-AuNCs) as a dual emission probe for ratiometric sensing of cyanide in tap water and cyanogenic glycoside-containing plants. The reduction of gold ion precursor with lysozyme generated lysozyme-stabilized AuNCs (Lys-AuNCs); they were demonstrated to be self-assembled into nano-aggregates during the formation of AuNCs. The aggregated Lys-AuNCs were treated with glutaraldehyde, triggering the conversion of aggregated lysozymes into blue-emitting lysozyme nanoparticles (LysNPs) via the formation of C=N bonds. As a result, the AuNCs were well-distributed inside the LysNPs, as demonstrated by transmission electron microscopy and size-exclusion chromatography. The as-prepared LysNP-AuNCs exhibited two separate emission bands, large Stokes shift, excellent photostability, and salt stability. The presence of cyanide triggered the etching of AuNCs in LysNP-AuNCs, leading to the suppression of fluorescence resonance energy transfer (FRET) from LysNPs to AuNCs. The LysNP-AuNCs probe was implemented for FRET detection of cyanide with linear range of 3100 M. Additionally, the selectivity of the LysNP-AuNCs probe for cyanide toward other anions was remarkably high. The practicality of the proposed probe was evaluated by quantifying cyanide in tap water and monitoring the liberation of hydrogen cyanide from ground cassava roots.