Production of mannan oligosaccharide using β-mannanase produced by Aspergillus niger on palm kernel cake in solid state fermentation

• Main Authors: Tsai, Ching-Hsuan, 蔡靜萱
• Other Authors: Sheu, Shyang-Chwen
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/cmwjgq

碩士 === 國立屏東科技大學 === 食品科學系所 === 106 === Thailand, Malaysia, Indonesia and other Southeast Asian countries are the main production areas of palm oil. Palm kernel cake (PKC), a waste product of the palm kernel oil extraction process contains more than 70% mannan of non-starch polysaccharides and therefore can serve as an excellent low-cost substrate for mannan oligosaccharide production. Mannan oligosaccharide can be used by probiotics in animal intestine to improve the composition of flora, and can stimulate the secretion of cytokines and chemokines from macrophages to enhance the immune response. In Southeast Asia, PKC is usually added into feed to increase the quality and nutritional value, but there is no reference demonstrated the application of enzyme on PKC for mannan oligosaccharide production. The objective of this study is to produce mannan oligosaccharide using β-mannanase produced by Aspergillus niger on PKC in solid state fermentation. The proximate analysis of PKC showed it contained 68.69±0.08% of carbohydrate, 15.43±0.18% of crude protein, 10.03±0.05% of moisture, 4.54±0.11% of crude ash and 1.31±0.24% effect of enzyme content by adding guar gum and locust bean gum as co-factor. The highest enzyme activity was shown when adding 1.5% of both carbon source co-factors. After PKC was hydrolyzed by crude enzyme solution for 30 minutes, 53.38±3.24 mg/g of total sugar, 34.58±0.17 mg/g of reducing sugar and 26.51±0.18 mg/g of mannan oligosaccharide were obtained. Mannobiose and mannotriose were the main contents of mannan oligosaccharides in the hydrolyzed product. The utilization of PKC could be enhanced by production of mannan oligosaccharide using β-mannanase.