Evaluation the Stability and Antioxidant Activity of Curcumin-loaded Liposomes

• Main Authors: Chuang, Hui-Yu, 莊蕙伃
• Other Authors: Shih, Wen-Ling
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/79k6b2

碩士 === 國立屏東科技大學 === 生物科技系 === 106 === Research indicates that the liposomes systems as a carrier can improve the biological efficiency. It is known that curcumin extracted from turmeric is a fat-soluble polyphenolic substance, in this study, the thin-film hydration method was used to encapsulating curcumin by refined phospholipids to liposomes and the method was conducted to evaluate the stability of the liposomes and the activity of antioxidant activity by an accelerated test, UVB irradiation and cell culture assay. The instrument of laser diffraction particle size analyzer was used to measure the particles size and the distribution of the liposomes. After 28 days of storage, the average particle size showed insignificant differences between low spinning speed and medium spinning speed, while unstable particle size showed under high spinning speed. However, the particle size showed a dose dependent UVB irradiation that it will decrease in higher UVB irradiation. In cytotoxicity assay, the concentration of IC50 was 177.7 ug/ml and 59.49 ug/ml when the cells were treated with different concentrations of curcumin for 24 and 48 hours, and the concentrations of curcumin loading liposomes in different concentrations were 250.4 ug/ml and 80.93 ug/ml. As a result of the above, the significant differences between curcumin and curcumin-loaded liposomes elucidated that, it is beneficial for cells to increase cell viability after liposomes are coated with curcumin. In the reactive oxygen species assay, they were co-treated with curcumin and curcumin-loaded liposomes. The amount of ROS production was significantly reduced, which helped to reduce the oxidation pressure to protect cell. In the reactive oxygen species (ROS) assay, the co-treatment between H2O2 with curcumin and curcumin-loaded liposomes demonstrated a significant reduced on the amount of ROS production compare to the control. This result explained that curcumin and curcumin-loaded liposomes able to protect the cells from the oxidation stress.