| Summary: | 碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 106 === The cnidarians form the amazing coral reef ecosystem mutually with zooxanthellae, its foundation was based on the intracellular symbiosis. By endosymbiosis, zooxanthellae were capable of living in cnidarian host gastrodermal endoderm cells with large numbers. Traditionally, the pH of symbiosomal lumen was thought to be 6-7. However, a recently report by utilizing pH indicators indicated that the pH of symbiosomal lumen in coral cells is below 4, revealing that the regulatory mechanism of symbiosome acidification should be clarified. Therefore, this study hypothesized that the pH of symbiosomal lumen in different cnidarian were different. Through culturing the fresh-isolated zooxanthellae with the different medium, the most suitable pH range of different zooxanthellae were evaluated. In this study, a total of eight of cnidarians species, and one test bivalves. The current results indicate that the zooxanthellae from Aiptasia pulchella, Bolocera mcmurrichi, Fungiidae spp. and Sinularia polydactyla, which were can growth at 6.2 and 8.6. The immunofluorescence showed that V-ATPase D was not abundantly distributed on the symbiosomal membrane of coral cells, suggesting symbiosomes were not acidic vesicles.
Keyword：Cnidarians、Symbiosome、Zooxanthellae、pH
|
|---|
