Effect of Human Alcohol and Aldehyde Dehydrogenases on Wound Healing of Human Oral Mucosa and Skin

• Main Authors: YANG, YA-CHU, 楊雅筑
• Other Authors: LEE, SHIAO-PIENG
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/u6grc8

碩士 === 國防醫學院 === 牙醫科學研究所 === 106 === Oral mucosa and skin wounds are trauma that are inevitable in daily life. It is generally considered that the repair time of oral mucosa trauma is shorter than skin trauma in clinical, but the detail mechanism is not clear. The past study which has confirmed that heavy drinking will increase the risk of wound bleeding, affect the immune system as well as the wound healing processes. Moreover, inflammatory reaction and ethanol metabolism will produce oxidative stress in tissue trauma area. The oxidative stress will cause the cellular DNA damage, which not only affects wound repair, but also induces apoptosis or cancerization. Human alcohol dehydrogenase (ADH) is the principal enzyme for ethanol metabolism, and the product of ADH reaction, acetaldehyde, is further oxidized by aldehyde dehydrogenase (ALDH) to acetic acid, which finally enters the citric acid cycle to provide energy. In addition, ADH and ALDH also affect the process of retinol oxidizes to retinoic acid. The purpose of this study is to investigate (i) the effects of ethanol and acetaldehyde in wound healing of oral mucosa and skin tissues, (ii) the expression level of free radicals produced by both, and (iii) the possible mechanism of ADH and ALDH in wounds. I analyzed the distribution of ADH and ALDH in the oral mucosa and skin tissue by immunohistochemistry, and used cell line WS1, and the primary oral fibroblast, HGF, to study cell migration during different concentrations of ethanol and acetaldehyde by wound healing experiment. We also detect intracellular free radical by H2DCFDA. In results, at the same drug concentration, the migration rate of oral cell is faster than that of skin cell, and otherwise the treatment of ethanol and acetaldehyde decrease the degree of cell migration. This phenomenon may be related to the accumulation of ethanol and acetaldehyde and the generation of free radicals. The slowness of cell migration is positively correlated with time as well as alcohol and acetaldehyde concentration after the drug treatment. In conclusions, alcohol consumption would lead to the wound under greater oxidative stress environment, and the increasing ethanol and acetaldehyde may compete with the oxidation of retinol that affects the mechanism of wound healing.