| Summary: | 碩士 === 國立嘉義大學 === 生物農業科技學系研究所 === 106 === Protein hydrolysate (SPH) and alginic acid (AlA) are biostimulants that can promote plant growth, enhance plant tolerance to stressful environment. AIA is a polysaccharide isolated from brown algae. Previous studies showed that AIA could promot plant growth, increase crop yield, and act as an elicitor to trigger defense response against pathogen infection. In this study, a SPH prepared by microbial fermentation and AlA were used to treat target plants. Our results showed that Arabidopsis treated with SPH and AlA increased root length and lateral numbers under osmotic stress. Treatment of SPH and AIA can increase root length, lateral numbers and decreased electrolyte leakage under cold stress. Treatment with SPH and AlA also promoted Arabidopsis growth. Tomato plant treated with SPH and AlA exhibited increased number of flowers in a single inflorescence, and increased GPX antioxidant enzyme activity and increased protein accumulation included CAT, AOX , LhcbI and PR3. Brassica (Brassica rapa chinensis, variety Brassica) treated with SPH and AlA exhibited lower H2O2 accumulation and malonaldehyde (MDA) content. In summary, our results showed that SPH combined with AlA can promote plant growth and antioxidant enzyme activity.
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