Effect of Graptopetalum paraguayense E. Walther water extract on intestinal microbiota composition and intestinal barrier in high fructose diet mice

• Main Authors: Huang, Xin-Sen, 黃信森
• Other Authors: Wu, She-Ching
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/k7gaaz

碩士 === 國立嘉義大學 === 食品科學系研究所 === 106 === Intestinal microbiota dysbiosis is highly correlated with many diseases such as colorectal cancer, diabetes mellitus and obesity. Studies have shown that fructose can cause intestinal microbiota dysbiosis and intestinal barrier dysfunction and hence inflammation of the liver. Graptopetalum paraguayense E.Walther belongs to the family Crassulaceae is the perennially succulent plant. The previous study demonstrated that water extract of G. paraguayense E.Walther (WGP) can improve the liver lipid accumulation induced by fructose by blocking the TLR4/NF-"κ" B pathway. Therefore, this research further induces the C57BL/6J mice model with high fructose diet (HF, 60%) to investigate the protective effect and potential mechanisms of the WGP in the intestinal microbiota dysbiosis and intestinal barrier dysfunction. The results show that WGP decreased the weight of the mice and increased the relative organ weight such as liver and epididymal fat, and lower down to 4.54 and 1.06 g/100 g of b.w respectively. In histopathology, after liver H&E staining, it was observed that the accumulation of small fat droplets in liver cells in the high-dose group (500 mg/kg/b.w) was significantly improved and the nucleus intact was not damaged. Treatment with different dose WGP could reach up the average large intestine length of mice to 8.14% and gallic acid (0.5 mg/kg/b.w) treatment was increased by 8.54%. This shows that WGP and its active constituents gallic acid can reduce inflammatory intestinal pointers. HF can lead to increased concentrations of triglyceride (TG) and total cholesterol (TC) in mice serum, aspartate-transglutaminase (AST) and alanine-transglutaminase (ALT). The above parameters can be reduced to 91.89 mg/dL, 72.93 mg/dL, 39.58 U/L and 43.21 U/L respectively after high-dose WGP treatment. HF can cause inflammation of the liver inflammatory cytokine TNF-α, IL-6 and IL-1β increase, WGP treatment can inhibit 34.85, 40.69 and 38.09% of the cytokine production respectively. HF can reduce the Bacteroidetes/Firmicutes ratio of intestinal microbiota in mice. Treatment with WGP and gallic acid could raise the Bacteroidetes to 38.24 and 34.36%, and reduce the Firmicutes to 26.37 and 27.92%. The relative percentage of Tenericutes was increased to 8.35% after high-dose WGP treatment, which showed that WGP could make the intestinal microbiota composition of the high fructose diet mice revert to the same composition as the normal diet group. In addition, WGP can elevate the protein expression of tight junction protein occludin and claudin-1 and can increase protein expression to 1.04, 1.10 and 0.83 respectively after treatment with different dose WGP. The relative expression of claudin-1 protein increased to 1.80, 1.82 and 3.84 after treatment with different dose WGP, and increased to 1.47 with gallic acid treatment, and there were significant differences between the HF group. In summary, the WGP can improve the liver fat droplets accumulation, inflammatory cytokine generation, intestinal microbiota dysbiosis and enhance the intestinal barrier structure to reduce the intestinal permeability in mice treated with high fructose diet. The results were used as the basis for developing and improving the related health products of intestinal microbiota.