| Summary: | 碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 106 === In the past, we known that mechanical cyclic stretching can induce reorganization of cytoskeleton and changed the cell migration and invasion. Therefor we identified the cyclic stretch-regulated gene expression in human lung cancer cell lines. Human lung cancer cell line A549 and H322m were subjected to cyclic strain (the frequency set in 15%, 0.5HZ) on 0h, 4h and 24h. We compared invasion assay, migration assay, colony formation and microarray assay in stretching and non-stretching groups. The results showed that the cell invasion in A549 and H322m were increased after 4hr stretching (p-value < 0.05). The microarray data showed that CXCL1, FOS, CCL2, CCL20, BIRC3, and TNFAIP3 were significant differences after 4hr stretching (fold change >2 or <-2). These genes have been reported to enhance cell invasion and these genes were cyclic stretch-regulated genes. Pathway enrichment analysis have found that these genes might regulate cells invasion through the TNF signaling pathway.
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