| Summary: | 碩士 === 國立中央大學 === 化學工程與材料工程學系 === 106 === Tumors contain a small subpopulation of cells, i.e., cancer-initiating cells or cancer stem cells (CSCs), which exhibit stem cell properties, possess a self-renewing capacity and are responsible for tumor generation and metastasis. Cancer stem cells persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumors. Furthermore, it is expected to establish primary colon cancer cell lines in vitro, since patient specific colon cancer cell lines is in great advantages of developing patient specific therapy in clinical application.
We are developing a method of establishing cancer cell lines from primary patient colon cancer tissue. Colon cancer tissue is digested by collagenase to generate colon cancer tissue solution. Subsequently, primary colon cancer cell lines were established by (a) culture method on specific culture materials, and (b) the membrane migration method through Nylon mesh filter.1 We investigate which factor is more important to establish the cancer cell lines from minimum amount of colon cancer tissue and determine the optimal conditions to establish patient specific colon cancer cell lines from primary colon cancer tissues. The patient specific colon cancer cell line will be useful for the patient-specific therapy in the future.
In this study, we designed cell sorting dishes from two combined concepts, which are physical cues and biological cues. Different extracellular matrix (ECM) and cell binding domain oligopeptides (biological cues) having different elasticity (physical cues) are immobilized on the cell sorting dishes as biological cues and physical cues for capturing CSCs. Optimal ECM/ECM-derived oligopeptide and elasticity of the dishes for (a) establishment of the patient-specific cancer cell lines and (b) isolation or depletion of CSCs have been shown in this study. Moreover, CSCs identification is quantified by colony forming assay and/or tumor generation in serial xenotransplantation model.
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