Many-Reactant and Multi-Target Sample Preparation on Flow-Based Microfluidic Biochips

• Main Authors: Wu, Shi-Yu, 吳錫御
• Other Authors: Huang, Juinn-Dar
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/scqzp5

碩士 === 國立交通大學 === 電子研究所 === 106 === In most biochemical applications, sample preparation is an essential and crucial procedure. While biochemical reactions take place, reagents or reactants must be mixed in a specific ratio. During this process, different reagents or reactants are mixed in an appropriate sequence to achieve given target concentrations. In the sample preparation problem of how to reduce the cost of reactants is a research topic of concern. To solve this problem, several works have been proposed recently. According to previous works, compared with digital microfluidic biochips using the [1:1] mixing model, flow-based microfluidic biochips with various available mixing models can massively reduce the reactant cost of biochemical applications. Therefore, we are motivated to make good use of flow-based microfluidic biochips to deal with many-reactant and multi-target sample preparation problem. In this thesis, we propose an algorithm that can properly produce intermediate solutions that can be efficiently shared among target concentrations, which effectively reduces the amount of waste solutions and thus minimizes the overall cost during the process. Compared with the prior art that can only process one target concentration at a time, experimental results show that our algorithm can save the reactant cost by up to 26.7%.