Raman microspectroscopic investigation on the symbiotic yeast isolated from mycangium of stag beetles endemic to Taiwan

• Main Authors: Huang, Yu-Min, 黃祐敏
• Other Authors: Hamaguchi, Hiro-o
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/dy95kd

碩士 === 國立交通大學 === 應用化學系分子科學碩博士班 === 106 === In recent decades, finding sustainable and renewable energy resources has become an issue of global importance. Among the sustainable energy resources, biomass has been focused especially. Till now, it is known for producing ethanol by corn, sugarcane, etc. Utilization of biomass, such as cellulose and xylan, as the starting material for biofuel production has been gathering attention. In this work, we used yeast symbionts which is isolated from mycangium (organ for transporting microbial symbionts) of female stag beetles. Because larvae of stag beetle is wood-feeding. And, the yeast symbionts, which are transferred from mother stag beetles, are thought to play an important role in the process of wood digestion. In this research, 632 nm excitation light source was used , and yeast symbionts isolated from nine different species of stag beetles were measured. We found there is particle moving around inside the cell, called “dancing body”, existing among these nine species. Besides only focusing on “dancing body”, mapping measurements on single yeast cell were also performed. Then, through the Multivariate Curve Resolution (MCR) analysis, the spectral components were successfully extracted.