Development of an electrochemical impedance spectroscopy based label-free biosensor for protein serine/ threonine kinases

• Main Authors: Lee, Jhao-Hong, 李昭宏
• Other Authors: Yuan, Chiun-Jye
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/79k3g2

碩士 === 國立交通大學 === 生物資訊及系統生物研究所 === 106 === In human genome, more than 80 % protein kinases are belonged to serine/ threonine kinase. The dys-regulation of protein serine/ threonine kinases may lead to various diseases, such as cancers and mental disorder. The ways for activity assay could be divided into two groups, labeling and non-labeling methods. The methods of non-labeling have many advantages, such as free of modification and easy to preparation. However, there is risk in determining the analysis result because of non-specificity and the bias by operators. In this work, an electrode design was proposed to effectively reduce the technical errors and bias during operation. According to the results of electrode surface by EIS, the activity of active protein serine/ threonine kinases could be obtained. By the results, the measuring range were from 0.01 to 5 unit/ mL and the limitation of detection was 0.005 unit/mL. It proved that the electrode design was effective to increase the feasibility of the non-labeling ways to analyze the activity of protein serine/ threonine kinases.