Antibody conjugated MR-optical imaging agent (MnMEIO-(ISX)-CyTE777-mPEG nanoparticle) for in vivo and in vitro bio-imaging of oncoprotein intestine specific homeodomain on hepatocellular cancer

• Main Authors: Liu, Yu-Han, 劉昱含
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/wcnnq8

碩士 === 國立交通大學 === 生物科技學系 === 106 === Hepatocellular carcinoma or HCC is the most common primary liver cancer. Although detailed oncogenesis is unknown, cirrhosis is considered to be the highest risk factor for the development of HCC. About 80 to 90% HCC cases was reported to occurred in the setting of cirrhosis. Recent research suggests that intestine specific homeobox (ISX) is a proto-oncogene for hepatocellular carcinoma which is triggered by cirrhosis related cytokines IL-6. Also, ISX is reported to have regulator effect on multiple cancer related cell functions including proliferation, transformation and immunosuppression. Further research on ISX could be important for advancing knowledge about HCC oncogenic pathway and possible early diagnostic of hepatocellular carcinoma. Nanoparticles (MnMEIO-(ISX)-CyTE777-mPEG) with fluorescence dye and iron oxide nanoparticals that specifically targeting ISX homeodomain were synthesized. Diameter of this particle is identified as approximately 20 nm which is suitable to enter the cell. And was studied for in vivo and in vitro imagine property. The result demonstrated that this nanoparticle is able to target and mark ISX homeodomain in vitro and in vivo, and have confirmed functional on both optical and MR imiging contrast enhancement.