| Summary: | 碩士 === 國立成功大學 === 化學工程學系 === 106 === In order to overcome problems so as to extend the applicability of FRET, we combine the use of semiconductor quantum dots (QDs) and AC electric fields to develop AC QD-based FRET for facilitating FRET probing. Our research group recently discovers that FRET signals emitted from QDs can be greatly amplified and undergo instantaneous changes under the actions of AC fields. And what I found after series of experiments is that (1) Both FRET signal and QD emission can change after AC switch. QD’s response is opposite to FRET’s. (2) Such QD switch can have different responses, depending on how QDs are assembled by electric fields and responses of assembled QDs can be changed by AC field. (3) FRET efficiency can be manipulated by joint effects of QD assembly and AC field.
Key words: Quantum Dot, Fluorescence Resonance Energy Transfer, AC Electro-kinetics, DNA probing
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