| Summary: | 碩士 === 國立中興大學 === 生物醫學研究所 === 106 === GCIP (Grap2 and CyclinD1 interacting protein) is a helix-loop-helix leucine zipper protein without DNA-binding domain. Recent studies had been demonstrated that GCIP is a tumor suppressor that suppress cell growth, proliferation, migration in vitro and in vivo. In our previous studies, it suggested GCIP and MEK2 (Mitogen-activated protein kinase kinase 2) will interact directly. Our data also confirmed MEK2 would reduce the protein stability of GCIP and phosphorylate GCIP on serine site.
GCIP was reported the ability of inhibition of cancer cell metastasis. Based on that, our past research demonstrated GCIP-mediated metastatic inhibition is through neither epithelial - mesenchymal transition (EMT) nor the metastasis related genes, such as Rac1, Rho A. Importantly, GCIP protein can regulate ITGAV (Integrin αv) gene expression negatively. Therefore, we used the luciferase assay observed GCIP and c-Myc will jointly mediate the transcription of ITGAV on negative regulation. These results indicate GCIP can inhibit the growth and metastasis of cancer cells, and it can be designed as a new cancer treatment strategy of cancers.
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