| Summary: | 碩士 === 明志科技大學 === 化學工程系生化工程碩士班 === 105 === The alkane production is applied on a large scale in the chemical industry and others chemical application, such as Jet fuel, gasoline and lubricant. In recent years we are responsible for the pollution mainly from the alkane process (fractional distillation of crude oil and chemical synthesis) .Because it do not comform basic concepts of green chemistry, and chemical reaction make complicated with high cost. These disadvantages is so terrible that chemical pollution entry for nature slowly, and with even lowers resistance. Therefore, we improve to problame of traditional process. The study investigation deals with the production of medium chain fatty acid from the yeast Y. lipolytica and technologe of conversion to alkane. The focus is on the state in biofuel and biochemical production, as well as discussion of the most important biomass feedstocks, conversion technologiesan and metabolic pathway. Through the integration of experimental result into analyzing. The thioesterase are of biochemical interest because of their roles in fatty acid synthesis and their utilities in the bioengineering. In the research we constructed three thioesterase gene in the pYES vector, that encoded by the Umbellular californica (FatB1), Cinnamomum camphorum (FatB1) and Cuphea hookeriana (FatB2) genes, that catalyze the limiting step of fatty acid shunt, and use the gene electrotransfer what implants the vector into Δpox2+ΔLeu auxotrophy of the Y. lipolytica. Thus, based on the results obtained, it not only increase over the 50% accumulation of the medium chain fatty acid shunt but make to further the identification of genetically modified. However, in order to show to three crucial gene that transform to alkane by medium chain fatty acid. Our research further suggest that overpressing to transformable gene of Fatty acyl-CoA synthetase (fadD), Fatty acyl-CoA reductase (acr) and Fatty aldehyde decarbonylase (CER1) in Y. lipolytica, and result with rate of conversion of fatty acid were enhanced more. To analyze precisely the yield of fatty acid and alkane that indicate data by the Gas chromatography. By genetic engineering and microbial technology the research control the accumulation of fatty acid successfully and improve the rate of conversion.
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