Study on the dual roles of dendritic cells in pulmonary inflammation: tolerogenicity and immunogenicity

• Main Authors: Tzu-Hsuan Wong, 翁子軒
• Other Authors: Jau-Ling Suen
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/rj29dz

博士 === 高雄醫學大學 === 醫學研究所博士班 === 106 === BACKGROUD: Dendritic cells (DCs) play a key role in modulating immune response. This study disscuss about the two different features when DCs directing immune response: tolerogenic and allergenic. DESIGN: Murine bone marrow derived dendritic cells were treated with CoPP (HO-1 inducer). Explore the tolerogenic feature of DCs and regulatory T cell induction ability. On the other hand, the mechanism of the aryl hydrocarbon receptor (AhR) in the process of promoting inflammation of DCs and its role in the disease model were discussed. RESULTS: The CoPP-treated mouse bone marrow dendritic cells have a reduced overall maturation and the secretion of pro-inflammatory cytokines but increase IL-10, and induce a higher amount of regulatory T cells both in vitro and in vivo. CoPP treated DCs also reduce the inflammatory intensity in asthmatic animal models. In the mouse model that simulates human inhalation of air pollution molecules and triggers allergic asthma, we not only found that Indeno [1,2,3-cd]pyrene (IP), the main molecule of air pollution in southern Taiwan, acts as a cofactor for enhancing allergic response and increased oxidative stress, but also revealed the importance of the AhR signaling pathway in DCs. CONCLUSIONS: We demonstrate that CoPP-DCs have tolerogenic phenotype and further promote the differentiation of antigen-specific regulatory T cell populations. It has also been found that air pollution exposure does increase the prevalence of allergic asthma. These findings will help develop antigen-specific immunotherapy such as autoimmune diseases or allergic asthma.