Effects of scaffold porosity on angiogenesis and its potential application on hepatic fibrosis therapy

• Main Authors: Yi-Jhen Wu, 吳宜真
• Other Authors: Shyh Ming Kuo
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/zu5u26

博士 === 義守大學 === 電機工程學系 === 106 === In general, Porous 3D scaffold has merits of providing larger surface and interconnection for cell growth and thereafter to generate tissues. The properties, pore size, pore geometry and the interconnection of pores all affect the cellular growth and differentiation of culture cells. Till today, It is very difficulty to prepare big pore size （＞300 µm）and good interconnection 3D porous scaffolds, especially for collagen biomaterials. In this present study, we prepared collagen scaffolds with various pore sizes and geometries and to evaluate the effects on cultured cells, angiogenesis and liver fibrosis repair. The obtained results indicated that we could produce average 80 µm pore size and good interconnection 3D collagen scaffold. Furthermore, we could produce more large pore size, about 500 µm, collagen scaffold by using alginate microspheres as porogen materials. The prepared collagen scaffolds all with good interconnection and with 99% water content. The porosity could be varied by using different alginate microspheres porogen and with a range of 70% -90%. The cell culture results showed that ADSCs grew well on large pore size collagen scaffolds. The ADSCs number did not change significantly after ultrasound stimulus. From SEM observation, the cell number increased obviously on ADSCs/Liver cell/HUVECs co-culture groups after ultrasound stimulus. The co-cultured cells grew better on the small pore size/mixed pore size collagen scaffolds; besides, ultrasound stimulus would influence the growth of cells. From confocal microscopy observation, the ADSCs expressed un-differentiated CD49d protein expression after 28-d culturing. The SD rats had obvious angiogenesis on the collagen scaffolds that implanted in the dorsal site after 4-w ultrasound stimulus. The liver repair and angiogenesis also appealed on rats that fibrotic liver implanted with small pore size collagen scaffolds.