| Summary: | 碩士 === 輔英科技大學 === 醫學檢驗生物技術系碩士班 === 106 === Oral cancer is the fourth leading cause of cancer death for males in Taiwan. Epidemiological studies have indicated that co-administration of natural compounds with anticancer drugs can lead to increased therapeutic activity against various types of cancers. Benzyl isothiocyanate (BITC), one of the best studied members of the isothiocyanate family, has been found to exhibit prevention of cancers in laboratory animals and might also be chemoprotective in humans. However, the effects of BITC on tongue cancer remain unknown. In this study, we aimed to examine the early signaling effects of BITC on human tongue cancer cells SAS.
Firstly, we evaluated the effect of BITC on cell viability of SAS cells using MTT assays. The results showed that BITC significantly decreased the viability of SAS cells in a concentration-dependent manner. Next, we assessed the effect of BITC (5, 10 μM) on reactive oxidative species (ROS) using DCF-DA reagent. ROS induced by BITC in OSCC cells was also in a dose-dependent manner. The experiment explored the mechanism of death of BITC against SAS cells. It was found that atypical DNA fragments after BITC treatment. Wound healing assay demonstrated that BITC exhibited an inhibitory effect on the abilities of migration in SAS cancer cells. Above results showed that BITC can inhibit the growth and proliferation of human tongue squamous cell carcinoma cells. It is expected that BITC can be a novel drug for the malignant progression of tongue cancer.
|
|---|
