Coenzyme Q10 ameliorates testicular oxidative stress and improves spermatogenesis function in male mice model of chronic kidney disease

• Main Authors: ZENG, XIANG-TING, 曾湘婷
• Other Authors: LIU, CHIN-YU
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/4e72d3

碩士 === 輔仁大學 === 營養科學系碩士班 === 106 === The study showed that 53% of male patients with chronic kidney disease (CKD) stages 3-4 had hypogonadism which is one of the important factors for male infertility. In CKD patients, oxidative stress may increase, which may cause germ cells damaged, testicular inflammation, and apoptosis. They may affect testosterone biosynthesis and spermatogenesis and thus reduce the reproductive function. In the testosterone biosynthesis pathway, cholesterol is converted to progesterone in mitochondria to synthesis testosterone. Coenzyme Q10 mainly acts as an antioxidant in mitochondria. Therefore, the aim of this study is to explore the effect of coenzyme Q10 on testicular oxidative stress and reproductive function in the chronic kidney disease. Nine-week-old C57BL/6 male mice were randomly divided into five groups: normal control group (NC group), sham-operated group received corn oil (SC group), sham-operated group received coenzyme Q10 (SQ group), the CKD-operated group received corn oil (CC group) and the CKD-operated group received coenzyme Q10 (CQ group). Chronic kidney disease was induced by 5/6 nephrectomy and the coenzyme Q10 was dissolved in corn oil fed by gavage. After eight weeks feeding, mice were sacrificed. The results showed that the concentration of coenzyme Q10 both in serum and testis were decreased in the CC group, and increased in the CQ group. In testosterone biosynthesis pathway, steroidogenic acute regulatory (StAR) protein expression was decreased in the CC group and increased in the CQ group. The immaturity of testicular cells and poor sperm quality of CKD mice also improved after eight-week coenzyme Q10 feeding. In addition, glutathione peroxidase (GPx) and catalase (CAT) activity could increase in the CQ group. In summary, CKD mice with coenzyme Q10 supplement ation have increased StAR protein expression in testosterone biosynthesis pathway, antioxidant activity, better sperm quality and the testicular cells morphology than CKD mice without coenzyme Q10 feeding.