| Summary: | 博士 === 輔仁大學 === 化學系 === 106 === Psoriasis is a common chronic inflammatory skin lesion. It affects 1–3% of the population worldwide and will be frequent recurrence, but not contagious. It is mainly due to the aberrant regulation of immune system in the skin that causes abnormal proliferation of skin cells and becomes thick dander. Typical psoriatic skin lesions are reddish, thick, scaly plaques that can occur on multiple skin sites all over the body. Actually, the real pathogenic mechanisms of psoriasis are still under study, which involves multiple interactions between the immune system and the skin system. However, the current therapeutic effects are limited, and there are severe side effects, therefore, it is necessary and urgent to develop new drugs. Our previous studies and other reports have demonstrated that both soybean extract and chrysin have strong antioxidant and anti-inflammatory effects. Their potential applications in the treatment of inflammation of the skin have also been reported in this study, which may help decrease inflammation and inflammatory pain. Through in vivo studies, we found that topical application of imiquimod (IMQ), a toll-like receptor (TLR)7 agonist and potent immune system activator, can induce and exacerbate psoriasis. Our results revealed that soybean extract and chrysin could obviously attenuate imiquimoid-induced psoriasis-like skin lesions in mice, and improve the imiquimod-induced disrupted skin barrier function. We found that the topical application of soybean extract and chrysin prior to IMQ treatment significantly decreased trans-epidermal water loss (TEWL), erythema, blood flow speed, and ear thickness, while it increased surface skin hydration, and attenuated epidermal hyperplasia and inflammatory cell infiltration. Through in vitro experiments, we found that soybean extract and chrysin can reduce IL-22, IL-17A, and TNF-α-induced MAPK, NF-κB, and JAK-STAT activation in normal human epidermal keratinocytes. Most importantly, soybean extract and chrysin could reduce TNF-α, IL-17A, and IL-22-induced CCL20 and antimicrobial peptides and proteins (AMPs) expression in epidermal keratinocytes. These results indicate that soybean extract and chrysin has great potential as an anti-psoriatic agent and in the treatment of other inflammatory skin diseases.
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