| Summary: | 碩士 === 朝陽科技大學 === 環境工程與管理系 === 106 === Antrodia cinnamomea, a heart-rot wood decay fungus of stout camphor (Cinnamomum kanehirae Hayata), is an endemic fungal species in Taiwan. Recently, people are paying more and more attention to search medicines from natural products. It has been reported that many chemical components of A. cinnamomea carry functional properties like tumor suppression, immunological enhancement. However the application of these components for medical cure has to found on the knowledge of the toxic dose of these components. This research studied the mutagenicity and anti-mutagenicity of Antrocin, an extract of A. cinnamomea, via mammalian microsome reversion assay (Ames test) to find the safe dose range and to verify the anti-cancer property of Antrocin and its derived pure compounds.
The TA98 and TA100 strains of Salmonella typhimurium were used to conduct Ames test. Up to 100 µg/plate of Antrocin gave no suppression effect to the number of colony multiplied of these two strains. The mutagenicity test assessing the security of Antrocin and the anti-mutagenicity test assessing the function of Antrocin were therefore tested up to this dose of 100 µg/plate. No mutagenic potential was found in the security assessment for both of TA98 and TA 100 strains. For anti-mutagenicity assessment, the inhibition rate to TA100 strain exhibited by Antrocin was weak, only up to 24.3%, if S9 was not added, lower than previous studies of this laboratory on row extract of Antrodia cinnamomea cultivated from various methods. However the inhibition rate to TA98 strain was 33.9%, higher than previous studies of this laboratory on row extract of Antrodia cinnamomea, and approximately doubled, 66%, while the liver activating enzyme S9 is present.
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