Human Blood Compatibility of Bioinspired Zwitterionic Charge-bias Formulations and its Medical Applications

• Main Authors: Jheng-Fong Jhong, 鍾政峯
• Other Authors: Yung Chang
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/7jrxc6

博士 === 中原大學 === 化學工程研究所 === 106 === In the past research, it was found that the positive charge and negative charge ionic groups composed with equal proportions to form zwitterionic and pseudozwitterionic complex, the neutral charge surface provide great hemocompatibility and anti-fouling property. In this study will start from this basic argument, to discuss how the positive charge and negative charge ionic groups complex structure surface charge-bias level influence the plasma protein adsorption and blood cell adhesion. And the results of this study were applied to the design of leukocyte reduction filter and animal wounds dressing, this study will help establish the design guidelines for a new generation of blood contact materials. The first part of this research using self-assembled technology and atomic chain transfer radical polymerization, targeting on utilizing the zwitterionic group’s positive-bias and negative-bias to control the interfacial polymerization precisely. In addition, the interactions between the surfaces of zwitterionic charge-biases and plasma proteins or blood cells are studied systematically, which also defines the functionality of various interfaces. The second part of this research, by controlling the mix ratio of positive charge and negative charge monomer to produce the pseudozwitterionic charge-bias ePTFE membrane and via low pressure plasma induce surface modification, and applied to the wound healing dressing, to discover the interaction between the hemocompatibility and wound healing, then defend the function of different pseudozwitterionic charge-bias wound dressing. In the third part, extended the application of pseudozwitterionic charge-bias structure, by controlling pseudozwitterionic charge-bias on the PP nonwven precisely and discuss its blood compatibility, defend the interaction between the charge-bias and blood cell, then actually applied to human blood leukocyte reduction filter. Of these three-part, X-ray photoelectron spectroscopy was induced to estimate the ratio of negative and positive charging group. The adhesion of plasma protein, platelets, red blood cells and white blood cells on the interface with different ratios of zwitterionic-bias is observed to provide the design principle for developing the functional material with excellent hemocompatility in the future.