Anti-diabetic and Anti-inflammatory Effects of Flavokawain A and Its Related Molecular Mechanism (s) in Murine Skeletal C2C12 Cells

• Main Authors: SU, YI-JYUN, 蘇依君
• Other Authors: WU, SHU-JUNG
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/5dt5q5

碩士 === 嘉南藥理大學 === 保健營養系 === 106 === Piper methysticum (PM) in Taiwan, China and Japan, etc. PM is popularly used as traditional medicines and health foods for antioxidant, anti-inflammatory and anti-cancer effects. In this study, our aims were (i) to examine the anti-diabetic and anti-inflammatory effects of a PM bioactive compound, flavokawain A (FKA) in palmitate (PA)-induced murine C2C12 skeletal muscle cells, and (ii) examine the effect of FKA on insulin, Akt/PI3K, MAPKs and GLUT-4 molecular mechanism (s) in PA-induced cells. As 10 to 30 μM FKA significantly up-regulated the glucose absorption and increased the cell growth. 30 μM FKA significantly up-regulated the glucose up-take, down-regulated ROS formation, as well as decreased the expression of NFκB in PA-induced cells. Results showed that only PA-treated cells, cell viability reduced to 81.80%, but FKA protected the cells to 100%. Co-treatment of cells with PA plus 30 μM FKA significantly up-regulated GLUT-4, down-regulated the activation of JNK, p38 and ERK proteins as well as inhibited the formation of ROS and decreased the levels of NFκB.