| Summary: | 碩士 === 嘉南藥理大學 === 生物科技系 === 106 === Filamentous fungi produce secondary metabolites with distinct structures and various biological activities for a wide range in biotechnology, food, and medical fields such as antibacterial, antifungal, and antitumor activity and used in different fields. Neosartorya fischeri is a heat-resistant mold frequently reported to cause spoilage in fruit products. Acetylaszonalenin is found to be the major secondary metabolite in N. fischeri, and catalyzed by anaPS, anaPT as well as anaAT encoded by a putative acetylaszonalenin-biosynthetic gene cluster. Even though this gene cluster was identified in N. fischeri, no reports on bioactivities of acetylaszonalenin could be found until now. Cause the precursor, benzodiazepinedione of acetylaszonalenin biosynthetic pathway whose antitumor activity was verified, we conjecture that acetylaszonalenin might have antitumor activity. To elucidate the antitumor activity of acetylaszonalenin, we used human colorectal adenocarcinoma cells as antitumor test object. We performed cell viability assay with various concentrations of acetylaszonalenin purified from mycelial liquid culture of
N. fischeri. The results indicated that there is cytotoxicity when human colorectal adenocarcinoma cells were tested after 24- , 48- and 72-hour treatments. To better understand the molecular mechanisms involved in the response of human colorectal adenocarcinoma cells to acetylaszonalenin, microarray analysis was used to help identify significantly expressed genes that may contribute to the response. After obtaining a large-scale survey of the variations in gene expression patterns and gene ontology enrichment analysis, the p53 signaling pathway would be the first pathway to be clarified at present. These results could provide insight information into the molecular mechanisms of the antitumor activity of acetylaszonalenin.
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