Interaction of influenza viral nucleoprotein with hnRNP A2/B1 and its impacts on viral replication

• Main Authors: Cheng Kai Chang, 張程凱
• Other Authors: S. R. Shih
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/484y48

博士 === 長庚大學 === 生物醫學研究所 === 106 === Viral host interaction contributes to both viral replication and host defense mechanisms during infection. A cellular protein, heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1, was found to interact with influenza A virus NP protein by antibody precipitation assay and MALDI-TOF identification. The interaction was further confirmed by means of co-immunoprecipitation. In vitro binding assay using a series of truncated forms of hnRNP A2 recombinant proteins revealed that glycine-rich domain (GRD) in C-terminus of hnRNP A2 was responsible for the interaction. In vivo immunoprecipitation assay also demonstrated F412 and R422 residues and tail loop domain of NP contributed to the interaction between NP and hnRNP A2/B1. hnRNP A2/B1 was found colocalized with NP in the nuclei of virus-infected cells at 4-6 hrs post-infection under confocal microscopy. Influenza viral RNP activity decreased in hnRNP A2/B1 silence cell which means hnRNP A2/B1 play as a positive regulator for viral RNA replication. This finding demonstrated cellular protein hnRNP A2/B1 can modulate viral RNA synthesis through binding to influenza viral protein NP.