| Summary: | 碩士 === 長庚科技大學 === 健康產業科技研究所 === 106 === Breast cancer is one of the most common cancers among women in the world, Its incidence has also been increasing year by year. Therefore, there is an urgent need to develop effective and safe anti-tumor drugs. Licochalcone A, was isolated from the root of Glycyrrhiza glabra, had multifunctional effects of biological and pharmacological activities, including anti-bacterial, anti-tumor, and cell differentiation capabilities. Therefore, in this study we investigated the cytotoxic effects of licochalcone A on breast cancer cells and its related mechanisms, including apoptosis, cell cycle, the generation of oxidative stress, DNA damage, and autophagy in breast cancer cell MDA-MB-231. The result demonstrated that licochalcone A effectively suppressed cell proliferation, cell cycle, and reduced cell migration. Licochalcone A also modulated mitochondrial membrane potential, and induced oxidative stress, DNA damage. Licochalcone A activated Cleaved-caspase 3 and Cleaved-caspase 9, and induced cytochrome c from the mitochondria into the cytoplasm. We also found that licochalcone A increase autophagy via activated LC3B expression. These results suggest that licochalcone A might increase the effect of apoptosis and autophagy on MDA-MB-231 cells. Therefore, licochalcone A may be a promising target for development as a breast cancer agent.
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