Virus Surveillance in Bivalve Shellfish and the Aquatic Environments in Taiwan and the Methodology Research on Virus Concentration

• Main Authors: TSENG, YU-TING, 曾郁婷
• Other Authors: HSU, BING-MU
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/7s8q7g

碩士 === 國立中正大學 === 地球與環境科學研究所 === 106 === This study focused on the occurrences of the adenovirus, norovirus, sapovirus, and rotavirus from aquatic environments and shellfish. The water samples collected from PuZi river basin, wastewater from livestock and businesses associated to the river basin 22 Taiwan’s fishing ports, oyster breeding area. Moreover, we also collected the shellfish samples from oyster breeding area and tourist fish markets of fishing port. Adenovirus is frequently found in a variety of aquatic environments and the dominant serotype of that were human adenovirus 41 and porcine adenovirus 5. Statistical analysis showed the significant correction between the occurrence of porcine adenovirus in fish’s ports and the pig farming number from river basin associated to corresponding fish’s ports. Hence we considered the occurrence of porcine adenovirus is suitable as indicator organisms to monitor the stool pollution from the pig farms. According to seasonal detections, Norovirus was mainly found in winter, agrees with many literatures. The dominant genogroup of Norovirus was GII, which was the dominant genogroup detected in Taiwan’s clinical norovirus. Sapovirus was sporadically detected in livestock wastewaters, fishing port waters, and market shellfish of winter season. Rotavirus was sporadically detected in the PuZi river basin and livestock wastewaters of summer and autumn seasons. This study used the Mann-Whitney U test compare differences between the presence/absence of pathogens and water quality parameters. Results showed that the HPCs, coliform group, E. coli, turbidity, salinity, and dissolved oxygen of water samples containing adenovirus were significantly different to those of the negative samples（M-W U test, p < 0.05）. The statistical result showed significant difference on temperature, pH, and dissolved oxygen for the presence of norovirus；significant difference on pH for the presence of norovirus；no significant difference for the presence of rotavirus with any of the water quality parameters. In order to understand the status of adenovirus in aquatic environments and to investigate adenovirus recovery rate on different water concentration methods, this study designed the six different water concentration methods to solve it. The preliminary outcomes showed that the ultracentrifuge method had the best water recovery rate, but considered the convenience and time consuming, this study recommends the original filter concentration combined with PEG precipitation as the best water concentration method regrading aquatic adenovirus for Laboratory operation.