Development on the Miniaturized Impedance Measurement Module for Cancerous cells

• Main Authors: JIH-HAO YEH, 葉日豪
• Other Authors: Chun-Ping Jen
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/c54e98

碩士 === 國立中正大學 === 機械工程系研究所 === 106 === Cancer has become a worldwide disease, and increases rapidly in recent years. A precise treatment depends on the accuracy of the diagnostic technique, in which includes analysis of cell number and cellular morphology. This study presented the development on the microfluidic device utilizing the dielectrophoretic impedance measurement (DEPIM) method which explores the human lung cell lines. The microchip consists of interdigitated microelectrodes with the circle -on-line shape and a micro chamber in which both DEP concentrating and impedance sensing for cells inside. This micro device has been fabricated by standard microfabrication processes. A highly sensitivity impedance measurement circuit board was built for the chip. It provides the sinusoidal signals with the frequencies in a wide range, so that the experiments can be performed in a reliable excitation. The results show a linear relationship between the admittance variation and the cell number in the different cell lines. The significant difference in the slope of these characteristic lines were adopted to distinguish two different lung cells, including A549- human lung carcinoma cells and MRC-5- human normal lung epithelial cells. The device is a simple-to-operate with high sensitivity, rapid response, inexpensive and portable application for cancerous cells.