Enhancing Fluorescent Emission Contrast of Fluorophores Coated on Metallic Nanostruture with Dielectric Materials

• Main Authors: Di-Wei Song, 宋狄威
• Other Authors: KAN,HUNG-CHIH
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/7d4acw

碩士 === 國立中正大學 === 物理系研究所 === 106 === We investigate the effect of periodical silver nanostructures and silica nanostructures on fluorescent emission from Streptavidin-Cyanine 3-tagged(Cy3-tagged) molecules. We measured the fluorescent intensity of Cy3 molecules deposited on silver nanostructure and silica nanostructure arrays. We found that the fluorescent intensity was enhanced by nanostructures.We also varied the excitation laser power and characterize the fluorescent contrast. We observed that the contrast decreased with excitation power. We also calculate the near-field enhancement by tempest 6.0 for all kind of periodical nanostructures. Finally, we compare the result of simulation and experiment to find some relation between them.